Professor of Biology
University of North Carolina at Chapel Hill
Carrboro, United States
My laboratory has:
Purified the gene for histones.
Demonstrated phased histones
Demonstrated a FIX variant with increased FIX activity
Purified and cloned the vitamin K-dependent gamma-glutamyl carboxylase
Purified the gene for the vitamin K epoxide reductase
Discovered that the binding site for FIX was type IV collagen
Identified the gene and protein for the "Antidotal Enzyme for Vitamin K Reduction When and Individual has Warfarin Poisoning/
1. Birnstiel M, Telford J, Weinberg E, Stafford D. Isolation and some properties of the genes coding for histone proteins. Proceedings of the National Academy of Sciences of the United States of America 1974; 71: 2900-4.
2. Simpson RT, Stafford DW. Structural features of a phased nucleosome core particle. Proceedings of the National Academy of Sciences of the United States of America 1983; 80: 51-5.
3. Chang J, Jin J, Lollar P, Bode W, Brandstetter H, Hamaguchi N, et al. Changing residue 338 in human factor IX from arginine to alanine causes an increase in catalytic activity. The Journal of biological chemistry 1998; 273: 12089-94.
4. Wu SM, Cheung WF, Frazier D, Stafford DW. Cloning and expression of the cDNA for human gamma-glutamyl carboxylase. Science 1991; 254: 1634-
5. Li T, Chang CY, Jin DY, Lin PJ, Khvorova A, Stafford DW. Identification of the gene for vitamin K epoxide reductase. Nature 2004; 427: 541-4.
6. Cheung WF, van den Born J, Kuhn K, Kjellen L, Hudson BG, Stafford DW. Proceedings of the National Academy of Sciences of the United States of America 1996; 93: 11068-73.
7. Jin, D. Y., X. Chen, Y. Liu, C. M. Williams, L. C. Pedersen, D. W. Stafford, and J. K. Tie. "A Genome-Wide Crispr-Cas9 Knockout Screen Identifies Fsp1 as the Warfarin-Resistant Vitamin K Reductase." Nat Commun 14, no. 1 (Feb 14 2023): 828.